Monday, March 4, 2019

Obeserving bacteris and blood Essay

ObjectivesThere were two major goals of the Observing Bacteria and Blood prove. The starting was to establish a familiarity with the proper techniques for using a microscope. The experiment cogitate on both the basic components of a microscope (i.e. how to adjust the knobs and levers for the desired result) as well as how do decide the proper intention to use up for observing a specimen. The second goal major goal of this experiment was to practice observing live specimens ( yoghurt and blood).Procedures work on 1 Observation of fain sheers using the microscope. The observation of the 8 prep atomic number 18d slides was performed by making adjustments to the microscope (focus, condenser, light) in order to establish a clear image of the specimen. The slide was viewed with the 10x, 40x, and 100x marks and all observations were recorded in the tables below.Exercise 2&3 Observationof yoghourt and blood. The yogurt and blood slides were prep bed as suck upd in the Labpaq manual . The slides were notice using the 10x, 40x, and 100x objectives and all observations were recorded in the tables below.Observations/ResultsExercise 1SampleObservations crack up 1Part 2150x600x1500x (Oil Immersion)Amoeba proteusAnabaenaAscaris Eggse slideParameciumPenicillium w/conidiaYeastYogurt bacteriumExercise 2 & 3SampleObservations150X600x1500xEx 2Fresh YogurtEx 3Blood SmearPhotos/Drawings (Insert and label images here. If image file (JPEG or GIF) sizes are too large they may be uploaded separately.)Analysis/ adaptation Based on the observations detailed above, it can be concluded that the microscope is a powerful tool in the observation of organisms that are small in size. It was important to have a number of objectives of different magnifying power in order to be able to discern the level of magnification unavoidable to best study a specimen. In addition, the examination of the yogurt sample allowed for the observation of the varying shapes and arrangement of bacteriuml cells. The use of a microscope to prise the blood smear highlighted the many important components of human blood that are not evident by the naked eye.Application In playacting this experiment I have learned many important aspects of microbiology that obtain to healthcare. First, there is plenty of information that can be discovered by looking at a sample under the microscope size, arrangement, number of organism. For example, this information can be utilized in making an initial appellative on a patient sample in a infirmary laboratory. Second, some microorganisms can bebeneficial such as those bacteria that can be cultured from yogurt. Furthermore, these microorganisms can also be employ to build back a healthy population of intestinal bacteria in patients who have recently been taking antibiotics.Answers to the LabPaq Questions1) Questions A. Identifythe following parts of the microscope and describe the functionof each.a. Ocular- eyepiece transmits and magnifies the image form the objective electron lens to the eye.b.Body/tube-holds the eyepiece at proper distance from the objective lens and blocks light.c.Nosepiece- rotating mount that hold objective lens.d.Objective Lens- gathers light for the specimen.e.Mechanical Stage- holds the specimen.f.Apeture diaphragm control/disc- alters the amount of light that reaches the condenser. g.Lamp- produces the light.h.Coarse focus knob-brings objects into focal point of the objective lens.i.Fine focus knob- makes fine adjustments to focus the image.j.Arm-holds all of the opthalmic parts at a distance and aligns them.k.Clips-hold the specimen still on the stage.l.Base- supports the weight of all the microscope parts.Define the following microscopy termsFocus positions the objective lens at the proper distance from the specimen.Resolution Ability for the lens to show fine details of the object being spy.Contrast The unfairness of the back ground relative to the specimen.B. What is the purpose of immersion oi l? To instantly the light from the microscope directly to the slide and stop it from refracting. It creats a finer issue and brightness.Exercise 2 Observing Bacteria Cultures in Yogurt Questions A. string your observations of the fresh yogurt slide.B. Were there observable differences between your fresh yogurt slide and the prepared yogurt slide? If so, explain.C. Describe the four principal(prenominal) bacterial shapes.Cocci oval or spherical shaped. Bacillus are rod shaped. Spirillum are thick, rigid spirals. Vibrio are swerve or a comma shaped rod.D. What are the common arrangements of bacteria? Diplo oval shaped, found in pairs.Strepto are coccus that arange into chains. Staphylo are cocci that are arranged into irregular clusters, similar to grapes.E. Were you able to identify particular proposition bacterial morphologies on either yogurt slide? If so, which types?Exercise 3 Preparing andObservingaBloodSlide QuestionsA. Describe the cells you were able to see in th e blood smear.B. Are the cells you ascertained in your blood smear different than the bacterial cells you have observed? Why or why not?

No comments:

Post a Comment

Note: Only a member of this blog may post a comment.